ANALISIS TES IMUNOKROMATOGRAFI DAN ENZYME-LINKED IMMUNOSORBENT ASSAY UNTUK MENDETEKSI Helicobacter pylori DI PASIEN DISPEPSIA

Abstract

Many methods are available to diagnose Helicobacter pylori in patients with dyspepsia, including Enzyme-Linked Immunosorbant
Assay (ELISA) and recently used is immnochromatography test. Both of the test are non invasive method, but immunochromatography
test can be used in laboratory without semi automatic analyzer. The aim of this study was to compare the immunochromatography
and ELISA tests in detecting the possibility of Helicobacter pylori infection. A cross sectional study was done among 49 samples
with dyspepsia at Wahidin Sudirohusodo Hospital of Makassar and Prodia Laboratory from March to August 2008. Samples were
analyzed with SPSS 14 for Windows Program using Chi Square and Spearman correlation tests. Among 49 samples we found ELISA
and immunochromatography tests were positive in 5 samples, ELISA and immunochromatography tests were negative in 33 samples,
ELISA tests were borderline and immunochromatography tests were positive in 5 samples, and ELISA tests were borderline and
immunochromatography tests were negative in 6 samples. There was a good correlation between ELISA and immunochromatography
test with R = 1.000 and p = 0.000. Both results of the immunochromatography and ELISA tests showed high conformity. Both method
can be applied to diagnose Helicobacter pylori in patients with dyspepsia. Immunochromatography test can be an alternative method
in laboratories who do not apply semi automatic analyzer. The advantages of immunochromatography test can be used for diagnose
early infection of Helicobacter pylori.