ANTI-HIV DAN SUBTIPE HIV PADA PASIEN HEMODIALISIS

Retno Handajani, Mochammad Thaha, Mochamad Amin, Citrawati Dyah Kencono Wungu, Edhi Rianto, Pranawa Pranawa

Abstract


Anti-Human Immunodeficiency Virus (Anti-HIV) was performed from 100 plasma Chronic Kidney Disease (CKD) stage 5 patients
with continuous hemodialysis (HD) at the Hemodialysis Instalation Dr Soetomo hospital, Surabaya, Indonesia, using three (3) kind of
reagents: Tri-line HIV Rapid test Device from Acon for HIV 1/2/O as strips form, Foresight HIV 1/2/O Antibody EIA Test Kit from Acon
and Anti-HIV 1+2/Subtype O ELISA from Axiom. HIV RNA and HIV subtype were detected by Reverse Transcription Polymerase Chain
Reaction (RT-PCR) based on HIV gag region and analysis of DNA result. Seventy three % patients were hemodialysed twice in a week and
only 14% with duration more than five (5) years. Most of the patients (43%) were hemodialysed between 100−300 times. From the 100
plasma samples was obtained only one (1%) man patient plasma sample with positive anti-HIV. A weak positive of RT-PCR result was
not succeed to be sequenced for determining the HIV subtype. This cause was suspected due to low levels of HIV RNA in blood. The results
of this study was expected can be used as an additional management consideration of hemodialysis patients at the Hemodialysis Unit.


Keywords


Hemodialysis, anti-HIV, subtype HIV

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DOI: http://dx.doi.org/10.24293/ijcpml.v22i2.1124

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