KADAR TGF-β1 PLASMA DAN LIMFOSIT-T CD4+DI PENDERITA YANG TERINFEKSI HIV STADIUM I

Abstract

HIV infection and AIDS have been spread throughout the world and the number of patients continues to increase from year to year.
Indonesia is one of the countries with quite a high increase in the incidence of HIV and AIDS. The absolute number of CD4+T-lymphocytes
and percentage in HIV-infected patients can be used to determine the stage of the disease, and progression of the disease, as well as to
predict the onset of the opportunistic diseases. In certain circumstances sometimes it is difficult to determine clinically and the results of
the absolute number of CD4+ T-lymphocytes and its percentage are still high. So the examination of TGF-β1 is necessary for predicting
the disease course in the patient, because the increase of the disease progress is also accompanied by the increased levels of TGF-β1. The
purpose of this study is to know and to prove the existence of the correlation between plasma levels of TGF-β1 and the absolute number
percentage of CD4+T-lymphocytes in stage I HIV-infected patients. The research was carried out by a Cross sectional observational study,
the samples were derived from 41 stage I HIV-infected patients treated at the Outpatient Clinic of the Infectious Disease Intermediate
Care Unit (UPIPI) in the Dr. Soetomo Hospital from January to May 2012. The examination of TGF-β1 plasma was performed by ELISA
method, the number of absolute and percentage of CD4+T-lymphocyte were counted by immuno flowcytometry (BD FACSCaliburâ„¢). The
results were statistically analyzed using a Pearson product moment correlation test. It was shown that the TGF-β1 plasma levels in stage
I HIV-infected patients tended to increase, as well as the number and percentage of CD4+ T-lymphocytes which were also increased. The
result of this study revealed that the number of CD4+T-lymphocytes which were less absolute and more than 200 cells /μL showed no
correlation with the plasma levels of TGF-β1 in stage I HIV infected patients. However, there was a significant positive correlation between
the number of CD4+T-lymphocytes percentage with TGF-β1 plasma in stage I HIV infection.