PENGANGKAAN (KUANTIFIKASI) PERIKSAAN PULASAN GRAM DI BERBAGAI JENIS BAHAN PEMERIKSAAN

Abstract

In a clinical microbiology laboratory the Gram staining is used to classify bacteria on the basis of their forms, sizes, cellular
morphologies, and Gram reactions. Additionally it is a critical test for rapid presumptive diagnosis of infectious agents and serves to
assess the quality of clinical specimens. Several methods of Gram staining quantification are already applied: Canadian Coalition for
Quality in Laboratory Medicine (CCQLM), Clinical Microbiology Proficiency Testing (CMPT), and World Health Organization (WHO).
Each method consists of several criteria for quantification and its interpretation, such as neutrophil cell (polymorphonuclear cells),
squamous epithelial cell, and number of microorganisms. Those methods aren't limited in sputum specimen, but also could be used for
other specimen such as urine, vaginal discharge, and other body fluids. These methods are also could be used as screening for specimen
before it is continued into further testing. Even though there is several limitation for each method, quantification method of Gram
staining could be provide better diagnostic value in microbiology laboratory as an early detection in the examination to get better
diagnosis as well as treatment.